Dinucleotide repeat polymorphism (D16S285) on human chromosome 16
نویسندگان
چکیده
منابع مشابه
Dinucleotide repeat polymorphism at the human preproglucagon gene.
References: 1) Bell.G.I. etal. (1983) Nature 304, 368-371. 2) Weber.J.L. and May.P.E. (1989) Am. J. Hum. Genet. 44, 3 8 8 3 % . 3) Schroeder.W.T. et al. (1984) Cytogenet. Cell Genet. 38, 76-79. 4) Weber,J.L. etal. (1990) Nucl. Acids Res. 18, 4637. Source/Description: The polymorphic (TG)n repeat begins at base pair 3833 in intron D of the human interleukin 9 gene (IL-9) on chromosome 5 (1). The...
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PCR Conditions: PCR was performed in a total volume of 20 microliters with 200 ng of genomic DNA, 8 pmoles of each primer, 2 mM MgCl2, 200 mM dNTP, 50 mM KC1, 5 mM Tris-HCl, pH 8.3, 0.01% gelatin, 0.5 U Taq polymerase (Boehringer). Primer Ang5 was 5' end labeled with P 7-ATP with the resulting specific activity 0.1-0.05 /iCi/pmol. Amplification was for 35 cycles with denaturation at 94°C 1 min,...
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MendeUan Inheritance: Co-dominant inheritance was observed in a five-generation pedigree in which 113 individuals were typed. Other Comments: The PCR was performed using P-labeled GPR 112-1 and unlabeled GPR 112-2 for 30 cycles: denaturation at 94°C for 1 min; annealing for 55°C for 1.5 min; and extension at 72°C for 3 min. The PCR products were analyzed on a 5% denaturing polyacrylamide gel (F...
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Polymorphism. Multiallelic dinucleotide repeat (CA-microsatellite) polymorphism in bovine genomic DNA, detectable by polymerase chain reaction (PCR), designated RM074. Source and Description. Sequence was obtained by conventional dideoxy methods from an M13mp18 template. The template was isolated from a "library" of size-selected ( -300 bp) MboI restriction fragments of bovine DNA by screening ...
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References: 1) Oshima,A. et al. (1988) J. Biol. Chem. 263, 2553-2562. 2) Laureys.G. etal. (1988) Genomicsi, 224-229. 2/162 6/162 129/162 20/162 1/162 4/162 0.01 0.04 0.80 0.12 0.01 0.02 Source/Description: The clone VAW140 (1) was tested for CA repeats by Southern blot hybridization (2). One CA-hybridizing Hindm fragment was subcloned into mp8 in both orientations and sequenced at each end. A (...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1991
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/19.19.5449